Alkamide Content and Localization in Heliopsis longipes Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy

Juan Vázquez Martínez and Jorge Molina Torres

Te invitamos a leer el artículo "Alkamide Content and Localization in Heliopsis longipes Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy" publicado en "molecules" en el que colaboró el Dr. Jorge Molina Torres de Cinvestav Irapuato.

Autores:

Juan Vázquez Martínez and Jorge Molina Torres

Resumen:

The alkamide content and specific tissue localization in the cypselae of Heliopsis longipes were investigated using gas chromatography–electron ionization mass spectrometry (GC-EIMS) and multiphoton fluorescence microscopy (MPFM). GC-EIMS analysis identified two olefinic alkamides: affinin (spilanthol) and N-2-methylbutyl-2E,6Z,8E-decatrienamide. Microscopic analysis revealed that alkamides are localized within the cotyledons, and specifically compartmentalized in lipid bodies, highlighting their spatial organization. The linear unmixing of fluorescence emission fingerprints showed that affinin exhibits autofluorescence at 693 nm, corresponding to the red spectral region. This emission is attributed to the conjugated double bonds in its acyl chain. This study is the first to report on the presence and precise localization of alkamides in the cypselae of H. longipes.