Non-targeted metabolomics for origin differentiation of cured vanilla pods (Vanilla planifolia) by LC-MS and GC–MS
Artículo
Te invitamos a leer el artículo "Non-targeted metabolomics for origin differentiation of cured vanilla pods (Vanilla planifolia) by LC-MS and GC–MS" publicado en Microchemical Journal, a cargo del investigador Cinvestav Dr. José Juan Ordaz Ortiz y su equipo de trabajo de la UGA.
Autores: Moisés Guerrero-Esperanza/ Rebeca Hernández-Peña/ Jorge Molina-Torres/ José J. Ordaz-Ortiz
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Laboratorio de Metabolómica y Espectrometría de Masas de la Unidad de Genómica Avanzada del Cinvestav
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Laboratorio de Fitobioquímica de la Unidad Irapuato del Cinvestav
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Summary:
Non-targeted metabolomic profiling was conducted using LC-HRMS and HS-SPME-GC–MS on cured Vanilla planifolia pods from Madagascar, Indonesia, Mexico, Papua New Guinea, and Uganda. Multivariate analyses (PCA, PLS-DA, hierarchical clustering) revealed compositional differences among samples, with partial separation by geographical origin. PLS-DA models presented acceptable predictive performance (Q2 = 0.823 and 0.710 for LC-HRMS and GC–MS, respectively) with statistical support from permutation testing (p < 0.00005). Several potential discriminant compounds were preidentified, including vanillin, protheobromine, specionin, and terpinolene. These findings suggest that geographical origin and post-harvest conditions may influence the chemical profiles of cured vanilla pods and highlight the importance of integrating multiple analytical strategies and multivariate analyses in exploratory studies of vanilla authentication.